3502 genome sequence Search Results


3502 genome sequence  (ATCC)
96
ATCC 3502 genome sequence
Organization of the genes studied in the dnaK operon (A) and groE operon (B) of C. botulinum <t>strain</t> <t>ATCC</t> <t>3502</t> (20).. The inverted arrows indicate the CIRCE binding element of the negative regulator HrcA. PA indicates potential σA-dependent promoter.
3502 Genome Sequence, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3502+genome+sequence/HCT116-SLC35F2-KO-c2/pmc03126429-234-37-36
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3502 genome sequence - by Bioz Stars, 2026-09
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klenow fragment  (New England Biolabs)
99
New England Biolabs klenow fragment
Organization of the genes studied in the dnaK operon (A) and groE operon (B) of C. botulinum <t>strain</t> <t>ATCC</t> <t>3502</t> (20).. The inverted arrows indicate the CIRCE binding element of the negative regulator HrcA. PA indicates potential σA-dependent promoter.
Klenow Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3502+genome+sequence/Klenow+Fragment/custom%40m0212%4020056143
Average 99 stars, based on 1 article reviews
klenow fragment - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier

Image Search Results


Organization of the genes studied in the dnaK operon (A) and groE operon (B) of C. botulinum strain ATCC 3502 (20).. The inverted arrows indicate the CIRCE binding element of the negative regulator HrcA. PA indicates potential σA-dependent promoter.

Journal: Applied and Environmental Microbiology

Article Title: Important Role of Class I Heat Shock Genes hrcA and dnaK in the Heat Shock Response and the Response to pH and NaCl Stress of Group I Clostridium botulinum Strain ATCC 3502 ▿

doi: 10.1128/AEM.02633-10

Figure Lengend Snippet: Organization of the genes studied in the dnaK operon (A) and groE operon (B) of C. botulinum strain ATCC 3502 (20).. The inverted arrows indicate the CIRCE binding element of the negative regulator HrcA. PA indicates potential σA-dependent promoter.

Article Snippet: The primers for the six class I heat shock genes studied ( hrcA , grpE , dnaK , dnaJ , groES , and groEL ) and for the 16S rrn were designed based on the published ATCC 3502 genome sequence (EMBL accession number {"type":"entrez-nucleotide","attrs":{"text":"AM412317","term_id":"148287495","term_text":"AM412317"}} AM412317 ) ( 21 ) using the Primer3-web-0.4.0 Web interface ( http://primer3.sourceforge.net/webif.php ) ( ).

Techniques: Binding Assay

Growth curves obtained in the gene expression experiments of the C. botulinum ATCC 3502 wild type grown at 37°C (filled circles), the C. botulinum ATCC 3502 wild type exposed to heat shock to 45°C (open circles), and the hrcA mutant exposed to heat shock to 45°C (open squares). Time zero (t0) is the time when the calibrator sample was withdrawn in the mid-exponential growth phase (OD600 of 0.9 to 1.1). The arrow indicates the time of the heat shock, and the dashed lines indicate times of sampling: immediately and 20 min, 1 h, 2 h, and 5 h after heat shock and at the corresponding time points for the untreated culture. The error bars indicate the variations of three biological replicates.

Journal: Applied and Environmental Microbiology

Article Title: Important Role of Class I Heat Shock Genes hrcA and dnaK in the Heat Shock Response and the Response to pH and NaCl Stress of Group I Clostridium botulinum Strain ATCC 3502 ▿

doi: 10.1128/AEM.02633-10

Figure Lengend Snippet: Growth curves obtained in the gene expression experiments of the C. botulinum ATCC 3502 wild type grown at 37°C (filled circles), the C. botulinum ATCC 3502 wild type exposed to heat shock to 45°C (open circles), and the hrcA mutant exposed to heat shock to 45°C (open squares). Time zero (t0) is the time when the calibrator sample was withdrawn in the mid-exponential growth phase (OD600 of 0.9 to 1.1). The arrow indicates the time of the heat shock, and the dashed lines indicate times of sampling: immediately and 20 min, 1 h, 2 h, and 5 h after heat shock and at the corresponding time points for the untreated culture. The error bars indicate the variations of three biological replicates.

Article Snippet: The primers for the six class I heat shock genes studied ( hrcA , grpE , dnaK , dnaJ , groES , and groEL ) and for the 16S rrn were designed based on the published ATCC 3502 genome sequence (EMBL accession number {"type":"entrez-nucleotide","attrs":{"text":"AM412317","term_id":"148287495","term_text":"AM412317"}} AM412317 ) ( 21 ) using the Primer3-web-0.4.0 Web interface ( http://primer3.sourceforge.net/webif.php ) ( ).

Techniques: Expressing, Mutagenesis, Sampling

Relative expression ratios of hrcA, grpE, dnaK, dnaJ, groES, and groEL at different growth phases of C. botulinum ATCC 3502 wild type grown at 37°C compared to mid-exponential growth. The 16S rrn was used as a normalization reference. The error bars indicate the variations of three biological replicates. Relative expression ratios that differ significantly from 1 (P < 0.05) are marked with an asterisk.

Journal: Applied and Environmental Microbiology

Article Title: Important Role of Class I Heat Shock Genes hrcA and dnaK in the Heat Shock Response and the Response to pH and NaCl Stress of Group I Clostridium botulinum Strain ATCC 3502 ▿

doi: 10.1128/AEM.02633-10

Figure Lengend Snippet: Relative expression ratios of hrcA, grpE, dnaK, dnaJ, groES, and groEL at different growth phases of C. botulinum ATCC 3502 wild type grown at 37°C compared to mid-exponential growth. The 16S rrn was used as a normalization reference. The error bars indicate the variations of three biological replicates. Relative expression ratios that differ significantly from 1 (P < 0.05) are marked with an asterisk.

Article Snippet: The primers for the six class I heat shock genes studied ( hrcA , grpE , dnaK , dnaJ , groES , and groEL ) and for the 16S rrn were designed based on the published ATCC 3502 genome sequence (EMBL accession number {"type":"entrez-nucleotide","attrs":{"text":"AM412317","term_id":"148287495","term_text":"AM412317"}} AM412317 ) ( 21 ) using the Primer3-web-0.4.0 Web interface ( http://primer3.sourceforge.net/webif.php ) ( ).

Techniques: Expressing

Relative expression ratios of hrcA, grpE, dnaK, dnaJ, groES, and groEL at different time points after heat shock (HS) at 45°C compared to pre-heat shock, mid-exponential growth of the C. botulinum ATCC 3502 wild type (A) and the hrcA mutant at 37°C (B). The 16S rrn was used as a normalization reference. The error bars indicate the variations of three biological replicates. Relative expression ratios that differ significantly from 1 (P < 0.05) are marked with an asterisk.

Journal: Applied and Environmental Microbiology

Article Title: Important Role of Class I Heat Shock Genes hrcA and dnaK in the Heat Shock Response and the Response to pH and NaCl Stress of Group I Clostridium botulinum Strain ATCC 3502 ▿

doi: 10.1128/AEM.02633-10

Figure Lengend Snippet: Relative expression ratios of hrcA, grpE, dnaK, dnaJ, groES, and groEL at different time points after heat shock (HS) at 45°C compared to pre-heat shock, mid-exponential growth of the C. botulinum ATCC 3502 wild type (A) and the hrcA mutant at 37°C (B). The 16S rrn was used as a normalization reference. The error bars indicate the variations of three biological replicates. Relative expression ratios that differ significantly from 1 (P < 0.05) are marked with an asterisk.

Article Snippet: The primers for the six class I heat shock genes studied ( hrcA , grpE , dnaK , dnaJ , groES , and groEL ) and for the 16S rrn were designed based on the published ATCC 3502 genome sequence (EMBL accession number {"type":"entrez-nucleotide","attrs":{"text":"AM412317","term_id":"148287495","term_text":"AM412317"}} AM412317 ) ( 21 ) using the Primer3-web-0.4.0 Web interface ( http://primer3.sourceforge.net/webif.php ) ( ).

Techniques: Expressing, Mutagenesis

Growth curves of the C. botulinum ATCC 3502 wild type (open circles), hrcA mutant (open squares), and dnaK mutant (open triangles) at the indicated pH values and temperatures in buffered TPGY broth (A to F) or in TPGY broth with added NaCl (G and H). The error bars indicate the variations of three biological replicates.

Journal: Applied and Environmental Microbiology

Article Title: Important Role of Class I Heat Shock Genes hrcA and dnaK in the Heat Shock Response and the Response to pH and NaCl Stress of Group I Clostridium botulinum Strain ATCC 3502 ▿

doi: 10.1128/AEM.02633-10

Figure Lengend Snippet: Growth curves of the C. botulinum ATCC 3502 wild type (open circles), hrcA mutant (open squares), and dnaK mutant (open triangles) at the indicated pH values and temperatures in buffered TPGY broth (A to F) or in TPGY broth with added NaCl (G and H). The error bars indicate the variations of three biological replicates.

Article Snippet: The primers for the six class I heat shock genes studied ( hrcA , grpE , dnaK , dnaJ , groES , and groEL ) and for the 16S rrn were designed based on the published ATCC 3502 genome sequence (EMBL accession number {"type":"entrez-nucleotide","attrs":{"text":"AM412317","term_id":"148287495","term_text":"AM412317"}} AM412317 ) ( 21 ) using the Primer3-web-0.4.0 Web interface ( http://primer3.sourceforge.net/webif.php ) ( ).

Techniques: Mutagenesis

The cultures of the C. botulinum ATCC 3502 wild type (A), hrcA mutant (B), and dnaK mutant (C) grown for 24 h on TPGY plates in a temperature gradient in the Gradiplate W10 incubator.

Journal: Applied and Environmental Microbiology

Article Title: Important Role of Class I Heat Shock Genes hrcA and dnaK in the Heat Shock Response and the Response to pH and NaCl Stress of Group I Clostridium botulinum Strain ATCC 3502 ▿

doi: 10.1128/AEM.02633-10

Figure Lengend Snippet: The cultures of the C. botulinum ATCC 3502 wild type (A), hrcA mutant (B), and dnaK mutant (C) grown for 24 h on TPGY plates in a temperature gradient in the Gradiplate W10 incubator.

Article Snippet: The primers for the six class I heat shock genes studied ( hrcA , grpE , dnaK , dnaJ , groES , and groEL ) and for the 16S rrn were designed based on the published ATCC 3502 genome sequence (EMBL accession number {"type":"entrez-nucleotide","attrs":{"text":"AM412317","term_id":"148287495","term_text":"AM412317"}} AM412317 ) ( 21 ) using the Primer3-web-0.4.0 Web interface ( http://primer3.sourceforge.net/webif.php ) ( ).

Techniques: Mutagenesis